Optimized broad-range real-time PCR-based method for bacterial screening of platelet concentrates
نویسندگان
چکیده
منابع مشابه
Rapid screening method for detection of bacteria in platelet concentrates.
Public awareness has long focused on the risks of the transmission of viral agents through blood product transfusion. This risk, however, pales in comparison to the less publicized danger associated with the transfusion of blood products contaminated with bacteria, in particular, platelet concentrates. Up to 1,000 cases of clinical sepsis after the transfusion of platelet concentrates are repor...
متن کاملPlatelet Factor 3 Based-clotting Time Assay as a Quality Marker for Long-term Storage of Platelet Concentrates
Background: Platelets rapidly lose their qualities usually after 5 day of storage. Different standard methods have been recommended to check the quality of platelets during storage which some of them show better correlation with other quality markers during storage. The purpose of this study was to demonstrate if platelet factor 3 (PF3) assay could be an indicator of storage lesion and provide ...
متن کاملDiagnosis of bacterial endophthalmitis by broad-range quantitative PCR.
AIM To measure the bacterial genome in ocular fluids and to analyse the clinical relevance of infectious endophthalmitis. METHODS Nineteen ocular fluid samples (eight aqueous humour and 11 vitreous fluid samples) were collected from 19 patients with suspected bacterial endophthalmitis. Fifty ocular samples from uveitis patients were also collected along with 40 samples from patients without o...
متن کاملDetermination of bacterial load by real-time PCR using a broad-range (universal) probe and primers set.
The design and evaluation of a set of universal primers and probe for the amplification of 16S rDNA from the Domain Bacteria to estimate total bacterial load by real-time PCR is reported. Broad specificity of the universal detection system was confirmed by testing DNA isolated from 34 bacterial species encompassing most of the groups of bacteria outlined in Bergey's Manual of Determinative Bact...
متن کاملReal-time PCR-based method for assaying the purity of bacterial artificial chromosome preparations.
Large-scale genomic sequencing projects (such as that being undertaken for the human genome project) often prepare large insert (100–200 kb) libraries in bacterial artificial chromosome (BAC) vectors. Short insert libraries are then constructed from individual isolated BACs, and clones from the short insert libraries are randomly selected for sequencing to the requisite coverage. BAC DNA is fir...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Brazilian Journal of Biology
سال: 2021
ISSN: 1678-4375,1519-6984
DOI: 10.1590/1519-6984.229893